Havard Nordtveit and Edimilson Fernandes featured for West Ham’s Under-23 side on Monday evening.
Anthony Scully scored the home team’s goal in a 3-1 defeat against Middlesbrough at the London Stadium, where Reece Burke returned to action after injury.
James Husband put the visitors ahead before West Ham were twice denied by the woodwork, with Dan Kemp heading against the bar and Joe Powell’s header hitting the post.
Hayden Coulson made it 2-0 and Marcus Tavernier got Boro’s third before Scully pulled a goal back with a superb free-kick.